Journal: eLife

Article Title: The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack

doi: 10.7554/eLife.41301

Figure Lengend Snippet:

Article Snippet: Cell line ( Rattus norvegicus ) , Normal rat kidney (NRK) fibroblast cell , ATCC , ATCC: CRL-1570; RRID: CVCL_2144 , .

Techniques: Recombinant, Plasmid Preparation, Antibody Labeling, Software

Journal: Journal of Cellular and Molecular Medicine

Article Title: Conditioned mesenchymal stem cells attenuate progression of chronic kidney disease through inhibition of epithelial-to-mesenchymal transition and immune modulation

doi: 10.1111/j.1582-4934.2012.01610.x

Figure Lengend Snippet: Effects of conditioned MSCs on expression of α-SMA and fibronectin in renal interstitial fibroblast NRK-49F. NRK-49F cells were cultured without or with 15 ng/ml TGF-β1 alone or co-culture with conditioned MSCs and/or ascorbic acid 2- phosphate for 3 days. ( A ) Representative Western blot analysis and relative bar graph analysis for α-SMA and β-tubulin level. ( B ) Representative Western blot and relative bar graph analysis of fibronectin protein level in NRK-49F after various treatments. * P < 0.05 versus normal control; ** P < 0.05 versus TGF-β1 treated.

Article Snippet: Rat renal proximal tubular cells (NRK-52E) and normal rat kidney interstitial fibroblast cells (NRK-49F) were purchased from the Bioresource Collection and Research Center of the Food Industry Research Institute, Taiwan.

Techniques: Expressing, Cell Culture, Co-Culture Assay, Western Blot, Control

Journal: The Journal of Physiology

Article Title: RAAS inhibitors directly reduce diabetes‐induced renal fibrosis via growth factor inhibition

doi: 10.1113/JP277002

Figure Lengend Snippet: A , C‐terminal of fibronectin (FBN‐C) turnover marker secretion by HK‐2 proximal tubular epithelial cells on HG and RAASi treatment. B , type IV collagen formation biomarker (PRO‐C4) secretion by HK‐2 cells on HG and RAASi treatment. C and D , PRO‐C4 secretion by NRK‐49F cells treated with platelet‐derived growth factor (PDGF; C ) or connective tissue growth factor (CTGF; D ). Values are presented as means ± 95% confidence intervals; n = 6 wells/group; one‐way ANOVA followed by Bonferroniʹs multiple‐comparison post hoc test; * P < 0.05 vs . control; §§ P < 0.01 vs . HG.

Article Snippet: Human kidney 2 proximal tubular epithelial (HK‐2) cells (LGC Standards, ATCC Cat. No. CRL‐2190, RRID:CVCL_0302) were cultured in DMEM containing 5.5 mM glucose (Gibco, supplied by Life Technologies, Carlsbad, CA, USA) and normal rat kidney fibroblast (NRK‐49F) cells (LGC Standards, ATCC Cat. No. CRL‐1570, RRID:CVCL_2144) were maintained in DMEM containing 25 mM glucose (Gibco), both supplemented with 10% FBS (Gibco), 1% penicillin/streptomycin and 1% L‐glutamine, and incubated at 5% CO 2 and 37°C.

Techniques: Marker, Biomarker Assay, Derivative Assay

Journal: The Journal of Physiology

Article Title: RAAS inhibitors directly reduce diabetes‐induced renal fibrosis via growth factor inhibition

doi: 10.1113/JP277002

Figure Lengend Snippet: A , representative picture of platelet‐derived growth factor receptor β (PDGFR‐β) stained NRK‐49F cells. 1000× magnification; red, PDGFRβ; blue, nucleus; scale bar = 20 μm. B – D , representative pictures of phalloidin‐TRITC immunostained NRK‐49F cells (control, B ) treated with platelet‐derived growth factor (PDGF; C ) or connective tissue growth factor (CTGF/CCN2; D ). 1000× magnification; red, F‐actin; blue, nucleus; scale bar = 20 μm. E and F , alpha‐smooth muscle actin (αSMA) protein levels in NRK‐49F cells treated with PDGF ( E ) or CTGF/CCN2r ( F ) and RAASi. Representative gel image examples shown above the panels. Samples might be from different gels but were derived at the same time and processed in parallel. On each graph values are presented as means ± 95% confidence intervals; n = 6 wells/group; one‐way ANOVA followed by Bonferroniʹs multiple‐comparison post hoc test; * P < 0.05, *** P < 0.001 vs . control; § P < 0.05, §§§ P < 0.001 vs . PDGF or CTGF.

Article Snippet: Human kidney 2 proximal tubular epithelial (HK‐2) cells (LGC Standards, ATCC Cat. No. CRL‐2190, RRID:CVCL_0302) were cultured in DMEM containing 5.5 mM glucose (Gibco, supplied by Life Technologies, Carlsbad, CA, USA) and normal rat kidney fibroblast (NRK‐49F) cells (LGC Standards, ATCC Cat. No. CRL‐1570, RRID:CVCL_2144) were maintained in DMEM containing 25 mM glucose (Gibco), both supplemented with 10% FBS (Gibco), 1% penicillin/streptomycin and 1% L‐glutamine, and incubated at 5% CO 2 and 37°C.

Techniques: Derivative Assay, Staining